Abstract
We have isolated cDNA and genomic clones containing α- and β-tubulin genes from Trypanosoma brucei. Each clone has been mapped, and the identity of the tubulin genes has been established by cross-hybridization with cloned chicken tubulin genes and by hybridization-selection and translation of trypanosome tubulin mRNA. In contrast with the dispersed organization of tubulin genes in other organisms, trypanosome α- and β-tubulin genes are physically linked and clustered in tandem repeats of approximately 13–17 copies per haploid genome of alternating α- and β-tubulin sequences.
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