Abstract
Cell-culture methods that simplify the inherent complexities of the kidney have not sufficiently reproduced its true characteristics. Although reports indicate that organoid methodology surpasses traditional cell culture in terms of reproducing the nature of organs, the study of human kidney organoids have been confined to pluripotent stem cells. Furthermore, it has not yet progressed beyond the developmental state of embryonic kidney even after complicate additional differentiation processes. We here describe the kidney organotypic culture method that uses adult whole kidney tissues but mainly differentiates into tubular cells. This model was validated based on the retention of key kidney organotypic-specific features: 1) expression of Tamm-Horsfall protein; 2) dome-like organoid configurations, implying directed transport of solutes and water influx; and 3) organoid expression of neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) in response to nephrotoxic injury (i.e., gentamicin and cisplatin exposure). This 3D-structured organoid prototype of the human renal tubule may have applications in developing patient-specific treatments for kidney diseases.
Highlights
The global prevalence of chronic kidney disease is 13.4%, which represents a major cost burden to healthcare systems worldwide [1]
Normal human kidney cells exhibited morphologic characteristics similar to primary renal proximal tubular epithelial cells (RPTECs) (ATCC, PCS-400-010), forming tight epithelial layers when grown to confluency in 2D culture (Fig 1A)
Tubulocysts counts in human kidney tubular organoids exceeded those of primary RPTEC organoids
Summary
The global prevalence of chronic kidney disease is 13.4%, which represents a major cost burden to healthcare systems worldwide [1]. Patient management remains limited to reducing inflammation, optimizing cardiovascular risk, and providing supportive care. A better understanding of physiologic and pathophysiologic mechanisms involved in renal damage and repair will likely promote therapeutic advancements [2]. The human kidney harbors a number of cell types, and its architecture is complex, posing a challenge for in vivo studies. Two-dimensional (2D) cell-culture models have value in the study of renal pathophysiology, the superiority of three-dimensional (3D) culture models that simulate the in vivo environment is readily acknowledged [2].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
