Abstract
Based on morphological and molecular phylogenetic analyses, two new species, Tuber shidianense and T. calosporum, are described from southwest China. They are clearly different from any other taxon in the genus Tuber. Tuber shidianense belongs to the Puberulum group. It is characterized by whitish ascomata, pseudoparenchymatous peridium and globose ascospores with deep alveolar ornamentation. Tuber calosporum belongs to the Macrosporum group. It is recognized by yellow and brown ascomata, prosenchymatous peridium and large elliptic ascospores with low alveolate wall. Phylogenetic relationships and morphological differences between these two new species and their related species are discussed.
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