Abstract
Objectives: To study the effect of Tspan5 on the proliferation and migration of trophoblast cells. Materials and Methods: The authors silenced the Tspan5 expression in human choriocarcinoma cell line JEG-3 using RNAi, and then conducted the cell proliferation assay and scratch assay to detect the proliferation and migration of JEG-3 cells. Results: The present authors found that the JEG-3 cell proliferation and migration activity of interference group (Tspan5/1005) had decreased significantly. Tspan5/1005 moderately decreased FAK and AKT phosphorylation compared to the control group. In this study, the authors found that the proliferation and migration of choriocarcinoma cells were reduced after using RNAi technology to downregulate the expression level of Tspan5 expression. The level of Tspan5 expression was positively correlated with the proliferation and migration of choriocarcinoma cells. Conclusions: Tspan5 could play a biological role through the FAK/AKT signaling pathway.
Highlights
The inner cluster of cells of the blastocyst will develop into an embryo while the outer layer, known as the trophoblast, will absorb nutrients directly from the mother for embryonic growth
Trophoblastic cells are uniquely different from other cells in terms of their biological behavior, development, and histogenesis. They can be divided into two cell layers, the outer layer known as syncytiotrophoblast (ST) and the inner layer known as cytotrophoblast (CT)
The authors found that Tspan5/1005 moderately decreased future. Cytoplasmic protein tyrosine kinase (FAK) and AKT phosphorylation compared to the control group (Figure 4)
Summary
A fertilized egg will move towards the uterus and undergo a series of cell divisions. A solid mass of cells known as a morula will form and a cavity will appear, where blastocoelic fluid will begin to accumulate, indicating the formation of the blastocyst. The inner cluster of cells of the blastocyst will develop into an embryo while the outer layer, known as the trophoblast, will absorb nutrients directly from the mother for embryonic growth. Trophoblastic cells are uniquely different from other cells in terms of their biological behavior, development, and histogenesis. They can be divided into two cell layers, the outer layer known as syncytiotrophoblast (ST) and the inner layer known as cytotrophoblast (CT). The ST facilitates the circulation of nutrients between the mother and the fetus while functioning as the endocrine tissue of the placenta [1]. The ST secretes a variety of protein hormones, including hCG [2]
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