Abstract
Phage display and mirror-image phage display are commonly used techniques for the identification of binders that are specific to predefined targets. Recent studies demonstrated the effectiveness of next generation sequencing (NGS) by increasing the amount of information extracted from selections. This allows for a better analysis and increases the possibility to select effective binders. A potential downside to NGS analysis of phage display selections is the increased workload that is needed to analyze the obtained information. Here we report on the development of TSAT (Target Specific Analysis Tool), software for user-friendly and efficient analysis of peptide sequence data from next generation sequencing of phage display selections.
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