Tryptose phosphate broth as supplementary factor for maintenance of HeLa cell tissue cultures.

Abstract

SummaryA mixture of tryptose phosphate broth, 15 to 25%, maintenance solution (MS), 67.5 to 77.5%, and chicken serum, 7.5%, maintained HeLa cells in tissue culture for at least 10 days. During this period HeLa cells increased 3 to 5 fold in number. Furthermore, smaller quantities of ARD, AD and Type I poliomyelitis viruses could be detected and significantly more ARD virus could be propagated in HeLa cells maintained in this medium than in cells supported with maintenance solution and chicken serum alone.