Tryptic soy medium is feasible for the in situ preparation of standards containing small defined numbers of microbial cells

Abstract

A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strains.