Abstract
Trypsin is often used to detach adhered cell subculture from a substrate. However, the proteolytic activity of trypsin may harm cells by cleaving the cell membrane proteins. The present study shows that cellular uptake of fluorescent nanoparticles is remarkably increased within 24 h after trypsinization. These results highlight the trypsin-induced protein digestion, provoking leaky cell plasma membrane which leads to the strongly enhanced cellular uptake of the nanoparticles. To prevent this effect, one should expose cells to the nanoparticle (NP)-based fluorescent labels at least 48 h after trypsinization.
Highlights
Since the intracellular uptake of inorganic nanoparticles (NPs) has been discovered, NPs became a widely used tool for long-term fluorescent imaging of living cells [1,2,3]
It has been successfully shown that the intensity of the intracellular uptake efficiency and localization of NPs inside the cells are strongly dependent on a set of various factors related to (i) NPs: size [4,5], surface shape [5,6], charge [7,8], and composition of protein corona [9] as well as to (ii) cell physiology: mitosis phase [10] and cell proliferation [11]
A powder mixture mainly constituted by carbon fluoroxide (CFO) NPs and 3C-SiC porous nanostructures was formed
Summary
Since the intracellular uptake of inorganic nanoparticles (NPs) has been discovered, NPs became a widely used tool for long-term fluorescent imaging of living cells [1,2,3]. It has been successfully shown that the intensity of the intracellular uptake efficiency and localization of NPs inside the cells are strongly dependent on a set of various factors related to (i) NPs: size [4,5], surface shape [5,6], charge [7,8], and composition of protein corona [9] as well as to (ii) cell physiology: mitosis phase [10] and cell proliferation [11]. It is used to break peptide bonds of the cell membrane proteins, in particular proteins responsible for cell adhesion.
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