Trypsin Stimulates Integrin α5β1-dependent Adhesion to Fibronectin and Proliferation of Human Gastric Carcinoma Cells through Activation of Proteinase-activated Receptor-2

Satoshi Miyata,Naohiko Koshikawa,Hidetaro Yasumitsu,Kaoru Miyazaki

doi:10.1074/jbc.275.7.4592

Satoshi Miyata, Naohiko Koshikawa + Show 2 more

Open Access

https://doi.org/10.1074/jbc.275.7.4592

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Abstract

Trypsin is widely expressed in various non-pancreatic tissues at low levels and overexpressed in some types of human cancers. In the present study, we found that trypsin stimulates integrin-dependent adhesion and growth of MKN-1 human gastric carcinoma cells. MKN-1 cells expressed both proteinase-activated receptor-1 (PAR-1) and PAR-2, which are activated by thrombin and trypsin, respectively. Both trypsin and the PAR-2 ligand SLIGKV promoted integrin alpha(5)beta(1)-mediated adhesion of MKN-1 cells to fibronectin, and less effectively integrin alpha(v)beta(3)-mediated cell adhesion to vitronectin, but not that to type IV collagen or laminin-1 at all. Thrombin and the PAR-1 ligand SFLLRN promoted the cell adhesion to vitronectin more strongly than trypsin or the PAR-2 ligand, but not the cell adhesion to fibronectin at all. The cell adhesion-stimulating effect of the PAR-2 ligand was significantly reduced by the pre-treatment of cells with trypsin, indicating that the effect of trypsin is mediated by PAR-2 activation. The trypsin-stimulated cell adhesion to vitronectin, but not to fibronectin, was effectively inhibited by the G(i) protein blocker pertussis toxin, and both cell adhesions were completely inhibited by the Src kinase inhibitor herbimycin A. Furthermore, trypsin and the PAR-2 ligand stimulated growth of MKN-1 cells more strongly than thrombin or the PAR-1 ligand. These results show that trypsin regulates cellular adhesion and proliferation by inducing PAR-2/G protein signalings, and that the integrin alpha(5)beta(1)- and integrin alpha(v)beta(3)-dependent cell adhesions are regulated by different PAR/G protein signalings.

Highlights

Has been well recognized that proteinases capable of degrading extracellular matrix (ECM)1 proteins play a critical role in tumor invasion and metastasis [9]
First we examined effect of trypsin treatment on adhesion of the parent MKN-1 cells, which secrete neither trypsinogen nor trypsin, to a plastic plate precoated with various concentrations of the cell adhesion proteins fibronectin
Herbimycin A completely inhibited the stimulatory effects of trypsin and the proteinase-activated receptors (PARs)-2 ligand on both cell adhesions to fibronectin and to vitronectin (Fig. 7, A and B). These results showed that Src kinase is involved in the stimulation of both integrin ␣5␤1-dependent and integrin ␣v␤3-dependent cell adhesions by PAR-2/G protein signaling

Summary

Introduction

Has been well recognized that proteinases capable of degrading extracellular matrix (ECM)1 proteins play a critical role in tumor invasion and metastasis [9]. First we examined effect of trypsin treatment on adhesion of the parent MKN-1 cells, which secrete neither trypsinogen nor trypsin, to a plastic plate precoated with various concentrations of the cell adhesion proteins fibronectin. We examined the effects of trypsin, thrombin, and the PAR-1- and the PAR-2activating peptides as the PAR ligands, on adhesion of MKN-1 cells to fibronectin, vitronectin, type IV collagen, and laminin-1 (Fig. 4).

Results

Conclusion