Abstract
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE–cellulose chromatography, with an estimated yield of 11–15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.
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