Trypanosoma evansi: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates

Daniel K Masiga,Kariuki Ndung’U,Alison Tweedie,Andrew Tait,C Michael R Turner

doi:10.1016/j.exppara.2006.03.002

Trypanosoma evansi: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates

Daniel K Masiga, Kariuki Ndung’U + Show 3 more

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https://doi.org/10.1016/j.exppara.2006.03.002

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Journal: Experimental parasitology	Publication Date: Apr 19, 2006
Citations: 46

Affiliation: Kenyatta University, University of Glasgow, Kenya Agricultural Research Institute, Wellcome Centre for Molecular Parasitology

#Amplified Restriction Fragment Length Polymorphism #Random Amplified Polymorphic DNA + Show 8 more

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Abstract

We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses. AFLP accessed many more polymorphisms than RAPD. Cluster analysis of the AFLP data showed that 12 T.evansi isolates were very similar (‘type A’) whereas 2 isolates differed substantially (‘type B’). Type A isolates have been generally regarded as genetically identical but AFLP analysis was able to identify multiple differences between them and split the type A T. evansi isolates into two distinct clades.

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