Abstract
A new radioisotope technique for quantitatively determining the extent of cell lysis of trypanosomes is described. Trypanosoma brucei was pulse-labeled with [ 3H]leucine and then incubated at 37 C in lytic (human serum) or nonlytic (rabbit serum) media. The release into the incubation medium of radioactively labeled intracellular macromolecules was then determined. A linear relationship was found between the release of TCA-precipitable radioactivity and lysis, as determined by conventional hemacytometer counting. This method is used to quantitate the trypanocidal activity in normal human serum.
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