Abstract
AbstractParasites of the genusTrypanosomaare microorganisms that display wide morphological, biological and genetic variability. Here we present the first description of an isolate of the genusTrypanosomanaturally infecting the tickAmblyomma brasiliense. The ticks were collected from a specimen ofTayassu pecari(Queixada, white-lipped peccary) from the Itatiaia National Park, Itatiaia, Rio de Janeiro, Brazil. The isolate was characterized by molecular, morphometric and biological analyses. ATrypanosomaculture was isolated from crushed nymphal and adult ticks, propagated in the tick cell line IDE8 and maintained in L15B culture medium, incubated at 32 °C. The isolate grew well in L15B medium at 30, 32 and 34 °C but not at lower or higher temperatures. The culture remained stable in axenic L15B medium at 30 °C. Cryopreserved cultures retained viability after cryopreservation in liquid nitrogen. Growth in axenic medium and developmental forms of the trypanosomes were analysed. Analysis of the 18S rDNA region confirmed the authenticity of this new species and the nucleotide sequence was deposited in Genbank. The species was namedTrypanosoma amblyommisp. nov. strain C1RJ. Characteristics related to pathogenicity, involvement with vertebrate hosts, epidemiology, developmental cycle and transmission mechanisms are still unknown. Therefore, further studies are necessary to understand the aspects of the biological cycle ofT. amblyommisp. nov.
Highlights
Members of the genus Trypanosoma belong to the family Trypanosomatidae and present a complex taxonomic classification due to their wide morphological, biological and molecular variation
We recently reported isolation of a novel trypanosome, Trypanosoma rhipicephalis sp. nov, from Brazilian Rhipicephalus microplus ticks removed from cattle (Marotta et al 2018)
This study describes an isolate of the genus Trypanosoma naturally infecting Amblyomma brasiliense ticks parasitizing the white-lipped peccary Tayassu pecari, characterized through molecular, morphological and biological analyses
Summary
Members of the genus Trypanosoma belong to the family Trypanosomatidae and present a complex taxonomic classification due to their wide morphological, biological and molecular variation. Several species of the genus Trypanosoma are aetiological agents of diseases transmitted to humans and animals, stimulating interest in these protozoans (Hoare, 1972; Haag et al 1998). This study describes an isolate of the genus Trypanosoma naturally infecting Amblyomma brasiliense ticks parasitizing the white-lipped peccary Tayassu pecari, characterized through molecular, morphological and biological analyses. A. brasiliense are aggressive towards humans, their vector capacity for aetiologic agents of diseases transmitted to humans or animals is unknown (Aragao, 1936; Sanches et al 2008)
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