Abstract
Only when Aquilaria spp. or Gyrinops spp. trees are wounded, due to insect attack, or microbial invasion, agarwood can be successfully induced. In the present study, a fungus which can induce agarwood formation efficiently was isolated and a suitable method for its application to induce agarwood formation was developed. Rigidoporus vinctus was isolated from the inner layers from infectious A. sinensis trees. When the fermentation liquid of fungi inoculated back to A. sinensis tree, agarwood was found to be induced. In addition, a novel method called trunk surface agarwood-inducing technique (Agar-Sit) was developed to produce agarwood with R. vinctus. The alcohol soluble extract content of the agarwood, up to 38.9%, far higher than the requirement (10%) in Chinese Pharmacopoeia and the six characteristic compounds of agarwood used as Chinese Medicinal Materials were all detected. Their relative percentages of the sesquiterpenes in the essential oil were 22.76%. This is the first report of the Agar-Sit and also the application of R. vinctus in agarwood induction. According to the results, when the combination of Agar-Sit and R. vinctus is used agarwood can be induced with high yield and good quality.
Highlights
Agarwood, the resinous wood of Aquilaria spp. or Gyrinops spp. trees [1,2,3], is highly valued for its extensive use in medicine, perfumes, and incense across Asia, Middle East and Europe [4]
The results show that the six characteristic peaks corresponding to the six typical components were identical with those in the typical chromatogram presented in Chinese Pharmacopoeia (Fig 4E)
The discovery of the isolate of R. vinctus was due to the observation that some wild fungi could enter the inner of wood and induce agarwood formation
Summary
The resinous wood of Aquilaria spp. or Gyrinops spp. trees [1,2,3], is highly valued for its extensive use in medicine, perfumes, and incense across Asia, Middle East and Europe [4]. Agarwood is one of the famous traditional medicine for sedative, carminative, and antiemetic effects in China, produced from Aquilaria sinensis. The wild resources of Aquilaria spp. and Gyrinops spp. are coming to be endangered and has been placed in the Appendix II list of the Convention on International Trade in Endangered Species of Wild Fauna and Flora since 2004 [5,6]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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