Abstract
Introduction TRPVs are a group of receptors with a channel activity predominantly permeable to Ca2+. This subfamily is involved in the development of gastrointestinal diseases such as ulcerative colitis (UC). The aim of the study was to characterize the gene and protein expression of the TRPV subfamily in UC patients and controls. Methods We determined by quantitative PCR the gene expression of TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6 in 45 UC patients (29 active UC and 16 remission UC) and 26 noninflamed controls. Protein expression was evaluated in 5 μm thick sections of formalin-fixed, paraffin-embedded tissue from 5 customized severe active UC patients and 5 control surgical specimens. Results TRPV2 gene expression was increased in the control group compared with active UC and remission patients (P = 0.002 and P = 0.05, respectively). TRPV3 gene expression was significantly higher in controls than in active UC patients (P = 0.002). The gene expression of TRPV4 was significantly higher in colonic tissue from patients with remission UC compared with active UC patients (P = 0.05) and controls (P = 0.005). TRPV5 had significantly higher mRNA levels in a control group compared with active UC patients (P = 0.02). The gene expression of TRPV6 was significantly higher in the colonic tissue from patients with active UC compared with the control group (P = 0.05). The protein expression of TRPV2 was upregulated in the mucosa and submucosa from the controls compared with the UC patients (P ≤ 0.003). The protein expression of TRPV3 and TRPV4 was upregulated in all intestinal layers from the controls compared with the UC patients (P < 0.001). TRPV5 was upregulated in the submucosa and serosa from the controls vs. UC patients (P < 0.001). TRPV6 was upregulated in all intestinal layers from the UC patients vs. controls (P ≤ 0.001). Conclusion The TRPV subfamily clearly showed a differential expression in the UC patients compared with the controls, suggesting their role in the pathophysiology of UC.
Highlights
TRPVs are a group of receptors with a channel activity predominantly permeable to Ca2+
Inflammatory bowel disease (IBD) is a group of diseases that comprises Crohn’s disease (CD) and ulcerative colitis (UC); both disorders are characterized by disturbances in the immune system and abnormal function of the gastrointestinal tract [1, 2]
TRPV2 gene expression was increased in the control group compared with active and remission UC patients (P = 0:002 and P = 0:05)
Summary
TRPVs are a group of receptors with a channel activity predominantly permeable to Ca2+ This subfamily is involved in the development of gastrointestinal diseases such as ulcerative colitis (UC). The gene expression of TRPV4 was significantly higher in colonic tissue from patients with remission UC compared with active UC patients (P = 0:05) and controls (P = 0:005). The gene expression of TRPV6 was significantly higher in the colonic tissue from patients with active UC compared with the control group (P = 0:05). In a model of TRPV2-deficient mice, the severity of DSSinduced colitis was lower in macroscopic, microscopic, and immunohistochemical levels in comparison with wild-type animals [7] These findings could be an effect from a reduced recruitment of macrophages to inflamed tissue, but it has been suggested that they could rely on the fact that TRPV2 participates in the regulation of the number and function of Th and Tc cells [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
