Troxerutin Delays Skin Keratinocyte Senescence Induced by Ionizing Radiation Both InVitro and InVivo.

Abstract

With the increasing demand for beauty and a healthy lifespan, studies regarding anti-skin aging have drawn much more attention than ever before. Skin cellular senescence, the primary cause of skin aging, is characterized by a cell cycle arrest in proliferating cells along with a senescence-associated secretory phenotype (SASP), which can be triggered by various internal or external stimuli. Recent studies have made significant progress in the fields of anti-senescence and anti-aging. However, little is known about the roles and functions of natural compounds, particularly flavonoids, in skin cellular senescence studies. In this study, using strategies including ionizing radiation (IR), senescence-associated β galactosidase assay (SA-β-Gal), immunofluorescence (IF), flow cytometry, PCR array, as well as invivo experiments, we investigated the effects and roles of troxerutin (Trx), a natural flavonoid, in skin keratinocyte senescence. We found that Trx delays skin keratinocyte senescence induced by IR. Mechanistically, Trx protects the skin keratinocyte cells from senescence by alleviating reactive oxygen species (ROS) accumulation, mitochondrial dysfunction, and DNA damage caused by IR. In addition, Trx was also proved to relieve skin senescence and SASP secretion invivo induced by IR stimulation. Altogether, our findings pointed to a new function of Trx in delaying stress-induced skin keratinocyte senescence, and should thus provide theoretical foundations for exploring novel strategies against skin aging.