TROSATIVE STRESS PARAMETERS IN COLON CANCER TUMOR, ADJACENT AND HEALTHY TISSUE

Branko Branković,Gordana Kocić,Zoran Krivokapić,Dušica Pavlović,Dejan Petrović,Andrej Veljković,Ivana Stojanović,Boris Đinđić,Goran Stanojević,Milica Nestorović

doi:10.5633/amm.2016.0107

Abstract

Colorectal cancer is one of the most frequent human malignant diseases and one of the most common causes of malignant diseases death. Oxidative and nitrosative stress have an important role in cancer initiation and propagation. That is why this study is focused on the determination of oxidative and nitrosative stress markers in tumor, adjacent and health tissue, which are important for the estimation of tumor proliferative and angiogenic potential. The study encompassed 50 patients who underwent surgery due to colorectal cancer. In the tissue samples from resected colon preparation (tumor, adjacent and healthy tissue, at least 10 cm distant from tumor), oxidative and nitrosative stress markers, malondialdehyde (MDA) and nitric oxide (NO) were determined. The obtained results prove the presence of oxidative stress in tumor tissue. Highly significantly (p<0.001) increased MDA concentrations in both tumor and adjacent tissue (12.43±9.39 and 11.57±5.56 nmol/mg proteins) compared to healthy one (7.25±5.52) reflect higher tumor aggressiveness and metastatic capacity. Higher NO concentrations in adjacent tissue (85.100±37.972 nmol/mg prot.) compared to the tumor one (58.608±22.789) point out high angiogenic potential of tumor surrounding tissue, which could have the clinical importance in the assessment of tumor invasiveness and the probability of local recurrence. In conclusion, the determination of the intensity of reactive oxygen and nitrogen species generation in tumor and adjacent colon tissue of patients with colorectal carcinoma could be useful in the estimation of the cancer invasive and metastatic capacity related to the prognosis of the disease and the choice of adjuvant therapy.

Highlights

Colorectal cancer (CRC) is one of the leading causes of cancer morbidity in world population, with the highest incidence rates in western coun-tries [1]
It is still not clear what is the initial event that leads to the activation of the cascade of the mechanisms underlying the transformation of normal colon mucosal cell into neoplastic one, there is a growing body of evidence that reactive oxygen and nitrogen species have important roles in cancerogenesis [18,19,20]
Tumor redox microenvironment is a critical factor in cellular signaling and reactive oxygen spe44 cies (ROS) play important roles as intracellular signaling molecules which regulate key cellular functions, such as proliferation, differentiation, growth, and apoptosis through cellular signaling

Summary

Introduction

Colorectal cancer (CRC) is one of the leading causes of cancer morbidity in world population, with the highest incidence rates in western coun-tries [1]. The determination of the intensity of reactive oxygen and nitrogen species generation in tumor and adjacent colon tissue of patients with colorectal carcinoma could be useful in the estimation of the cancer invasive and metastatic capacity related to the prognosis of the disease and the choice of adjuvant therapy. Trosative stress parameters in colon cancer tumor, adjacent...

Results

Conclusion