Abstract

It is demonstrated by gel-filtration, analytical ultracentrifugation and disc gel-electrophoresis that urea can dissociate the relaxing protein preparation into at least two different protein fractions, and that a single protein component similar to the relaxing protein can be formed from troponin and tropomyosin preparations. Thus a proposal of Ebashi and Kodama that the relaxing protein is a complex of troponin and tropomyosin is supported.

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