Abstract
In our investigation,we used short-time model of myocardial infarction of rats induced by high dose of isoproterenol (ISP). We investigated cardiac troponin T blood level (cTnT) and histological characteristics of rat myocardium. ISP, single, intraperitoneal dose 250 mg/kg was given to male, adult, Wistar rats (n=12). Rats were distributed depending on their body weight in subgroups: ISP I (BW 260-280g) and ISP II (BW 250-400g).Control group (n=9) was treated with intraperitoneal dose of 0,95% NaCl. Cardiac TnT was measured by electrochemiluminiscence (ECLA) sandwich immunoassay in rat serum 4 hours after ISP application. Rats' hearts were dissected and examined by qualitative histological method (HE). Statistical significance was set at 0,05. There was significant difference in cTnT of ISP II (p=0,0001) vs. control and ISP I (p<0,05) vs. control. Significant difference was between ISP I and ISP II subgroups (p<0.001). The accent of histological changes of myocardium was on nuclei of cell. Cells showed acidophilic changes and nuclei disappearance as signs of coagulative necrosis development. Extensivity of histological changes were different between ISP I and ISP II subgroup. Used dose of ISP induced development of myocardial necrosis in rats. Subendocardial portion of myocardium was more vulnerability than subepicardial portion. Rats of ISP II had more extensive histological changes than these in ISP I. Administered doses of ISP enabled cTnT utilization as a marker of myocardial necrosis.
Highlights
We’re witnesses of permanent changes in diagnosing and therapy of acute myocardial infarction patients
Permanent changes are result of experimental investigations and better understanding of molecular mechanisms occurred during myocardial necrosis development
Cardiac troponin T belongs to the proteins of contractile apparatus that are unique for cardiac muscle
Summary
We’re witnesses of permanent changes in diagnosing and therapy of acute myocardial infarction patients. Troponins are highly sensitive and specific markers of cardiac cell damage ( ) These contractile proteins are released from myocardium in proportion to the degree of tissue injury. There is so little information about cTnT blood level and histological finding of myocardium in ISP induced rat model of myocardial infarction. There isn’t wellstandardized short time animal model of rat myocardial infarction induced by ISP for studying of: a) histological and cardiac marker changes during myocardial infarction, b) cardioprotective and c) cardiotoxic drugs investigation. In this short time rat model, we examine histological characteristics of cardiac muscle damage and TnT blood level in rats hours after ISP administration
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