Tropical Atlantic marine macroalgae with bioactivity against virulent and antibiotic resistant Vibrio

Giselle Cristina Silva,Regine H Silva Dos Fernandes Vieira,Renata Albuquerque Costa,Fernando E Pessoa Nascimento,Jackson R Oliveira Peixoto,Pedro B De Macedo Carneiro

doi:10.3856/vol41-issue1-fulltext-17

Giselle Cristina Silva, Regine H Silva Dos Fernandes Vieira + Show 4 more

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https://doi.org/10.3856/vol41-issue1-fulltext-17

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Abstract

The antibacterial activity of ethanol, methanol, hexane and acetone-based extracts of the macroalgae Padina gymnospora (PG), Hypnea musciformes (HM), Ulva fasciata (UF) and Caulerpa prolifera (CP) was investigated. The disk diffusion method was used to evaluate the algae antimicrobial effect against standard strains of Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica and five virulent antibiotic-resistant strains of V. brasiliensis, V. xuii and V. navarrensis (isolated from the hemolymph of Litopenaeus vannamei). Ethanol extracts of PG and HM inhibited all Vibrio strains. E. coli and P. aeruginosa were only susceptible to ethanol extracts of PG. Among the methanol extracts, only UF was bioactive, inhibiting V. navarrensis. The observed inhibitory effect of ethanol extracts of PG, HM and UF against virulent antibiotic-resistant bacteria suggests these macroalgal species constitute a potential source of bioactive compounds.

Highlights

The antibacterial activity of ethanol, methanol, hexane and acetone-based extracts of the macroalgae Padina gymnospora (PG), Hypnea musciformes (HM), Ulva fasciata (UF) and Caulerpa prolifera (CP) was investigated
Investigations on bioactive compounds have mostly been focused on phanerogams (Nair & Chanda, 2007; Peixoto et al, 2011), but macroalgae have been shown to contain compounds capable of inhibiting bacterial growth, including that of well-known human pathogens (Taskin et al, 2007; Kolanjinathan & Stella, 2009)
The objective of this study was to evaluate the bioactivity of extracts of the marine macroalgae species Padina gymnospora, Hypnea musciformes, Ulva fasciata and Caulerpa prolifera against standard strains of Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica, and against antibiotic-resistant vibrios carrying virulence factors

Summary

Introduction

The antibacterial activity of ethanol, methanol, hexane and acetone-based extracts of the macroalgae Padina gymnospora (PG), Hypnea musciformes (HM), Ulva fasciata (UF) and Caulerpa prolifera (CP) was investigated. The objective of this study was to evaluate the bioactivity of extracts of the marine macroalgae species Padina gymnospora, Hypnea musciformes, Ulva fasciata and Caulerpa prolifera against standard strains of Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica, and against antibiotic-resistant vibrios carrying virulence factors.

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