Abstract
A special in-vitro protocol was elaborated enabling the production of potato single-node explant plantlets that can be used as objects for tropic studies. In light-grown plantlets, achievement of a full (90?) phototropic (PT) curvature required 75 to 120 min of continuous unilateral blue light irradiation or 120-135 min of gravitropic stimulation (GT). Time-lapse photography revealed that the curves describing PT and GT bending have a sigmoid shape. Continuous BL irradiation was necessary for the induction of continuous PT bending. If the BL was turned off after 30-50 min of PT stimulation, the bending gradually decreased and stopped in darkness after 25.0 ? 2.0 min. Within this period, curvature increased by 15.5 ? 1.5?. When the BL was turned off upon completion of PT bending (when the plantlets reached an angle of 90?), the plantlets entered the phase of fast straightening. The 90? PT curvature was significantly exaggerated in darkness by turning the jars from a vertical to horizontal position providing 120.74 ? 2.5? as the final curvature angle after two more hours in darkness.
Highlights
Light-grown potato plantlets derived from singlenode explants were observed to manifest vigorous photo- and gravitropic movements in vitro (Vinterhalter et al, 2010), offering a novel model system for tropic response studies to be established
There is no doubt that photo- and gravitropism are the most important tropic movements of higher plants
Most of the research was performed on the A. thaliana model system and perhaps it is time to shift our attention to other plant species
Summary
Light-grown potato plantlets derived from singlenode explants were observed to manifest vigorous photo- and gravitropic movements in vitro (Vinterhalter et al, 2010), offering a novel model system for tropic response studies to be established. To meet the needs of these studies, we had to optimize the protocol for the production of experimental material (single-node shoot explants) and to define experimental conditions supporting their fast and reproducible tropic responses. Sub-culturing was done by shoot dissection into single-node explants (SNE) where each explant contained one node carrying an axillary bud. After excision, these explants exhibited fast growth forming a complete, rooted plantlet in just 11-14 days
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