Trophic relationships of grassland ants based on stable isotopes

Abstract

As keystone species in many ecosystems, ants are important predators of insects and other invertebrates, which are fed mainly to the ant larvae. Honeydew is important in the diet of adult worker ants and some ant species collect seeds as food items. Differences in the natural abundance ratios of the stable isotopes of carbon and nitrogen were used to investigate the trophic relationships of eight species of ants in limestone grassland near Limerick, mid-west Ireland. During July 2005, samples of adult workers and larvae were collected from ten nests of each of eight sympatric species ( Myrmica ruginodis , M. scabrinodis , M. sabuleti, M. schencki, Leptothorax acervorum, Lasius flavus , L. niger , and Formica lemani) and analysed using Elemental Analysis–Isotope Ratio Mass Spectrometry (EA–IRMS). Myrmica ruginodis workers had the highest δ 15 N, and M. schencki the lowest δ 15 N. The δ 15 N spacing between these two species was 2.6‰, which represents a difference of almost one trophic level. High δ 15 N values for adult Lasius flavus were unexpected as they suggest a more predatory diet for L. flavus workers than is implied in the literature, or possibly a diet including Collembola which can have higher δ 15 N values than herbivorous arthropods. The results for Formica lemani indicate that its diet is more generalist than field observations and the literature suggest. The larvae of L. acervorum had the lowest values for δ 15 N and δ 13 C (probably reflecting a high lipid content) and the values for the workers were also low, suggesting that the food sources of this species, which are little known, are different from those of the other co-occurring ant species in the study.