Trophic niche partitioning of golden cuttlefish (Sepia esculenta) during ontogeny revealed by stable isotope analysis and DNA metabarcoding

Abstract

Cephalopods are becoming more important in global fishery. Understanding the trophic ecology partitioning during their ontogeny is critical to the stock assessment and fishery management. The golden cuttlefish (Sepia esculenta), one of commercially important cephalopod species has been found at different life-history stages that co-inhibit in the coastal waters of Qingdao, China. In the present study, the trophic ecology of S. esculenta was assessed by using stable isotope analysis (SIA: based δ13C and δ15N from muscle and liver tissue) and DNA metabarcoding of the stomach contents through high-throughput sequencing (HTS). SIA uncovered the variation in isotope niche and trophic position of S. esculenta during ontogeny. DNA metabarcoding showed a higher resolution of prey identification than before. The combined analysis of SIA and DNA metabarcoding indicated that diet shifts occurred in the ontogeny of S. esculenta. Paralaval and juvenile prefer zooplankton, while sub-adult and adult prefer demersal fishes. The standard ellipse area (SEAc) indicated that low isotope niche overlaps among paralaval, juvenile and sub-adult of S. esculenta, which reflect the squid foraging strategy during ontogeny. The study confirmed that S. esculenta resources can be partitioned into different life stages, suggesting a survival strategy to maintain population dynamics.