Trophic ecology of three marine polychaete species: Evidence from laboratory experiments using stable isotope (15N, 13C), fatty acid (NLFA) analyses, and C and N stoichiometry

Abstract

Marine polychaetes are a highly diverse taxon with a large variety of different feeding modes. A proper identification of the different diets of polychaete species and their trophic position in the food web is crucial for understanding their interactions in marine ecosystems as well as marine community dynamics. Since gut content analyses and feeding experiments are difficult to conduct in marine habitats, other methods using biochemical tracers may contribute to a better understanding of the trophic ecology of marine polychaetes. Here, we conducted laboratory experiments and used a combination of stable isotope (13C, 15N) and neutral lipid fatty acid (NLFA) analyses, as well as C and N stoichiometry to determine the feeding habits of widely distributed marine polychaete species, such as Eurythoe complanata, Platynereis massiliensis, and Syllis malaquini. Additionally, the impact of starvation on the stable isotope signatures was analyzed. Our data show that the trophic ecology of the three species differs from each other. Stable isotope and fatty acid analyses indicate that (a) E. complanata is mainly feeding on algae, cyanobacteria and to a lesser extent on fungi, that (b) S. malaquini is mainly feeding on algae and bacteria and that (c) P. massiliensis is mainly feeding on algae, especially diatoms. An analysis of the C:N ratio of the respective annelid species and their potential food sources corroborated these results. The combination of stable isotope signatures, fatty acid markers and stoichiometry of carbon and nitrogen is a useful tool to identify the diet and trophic position of marine polychaete species and provides more results about their feeding habits and their position in marine food webs.