Trophic ecology, diet and feeding behaviour of three bathyal sea anemones (Actiniaria: Cnidaria) in the Northwest Atlantic

Abstract

Sea anemones are often presented as a major component of benthic communities and described as ecologically important in benthic food webs. However, studies on the trophic ecology of deep-sea species are rare. Here, the feeding habits and dietary sources of three species of bathyal sea anemones (Actinostola callosa, Actinauge cristata, Urticina sp.) from the Northwest Atlantic were studied in a multi-faceted approach including stable isotopes, gastrovascular contents, lipid and fatty acids analysis and observations in a mesocosm. Stable isotope analysis showed that A. callosa sits at a slightly lower trophic level than A. cristata and Urticina sp. and that the two latter species rely on different carbon sources at roughly the same trophic level. The gastrovascular cavity contents and mesocosm study revealed that all three species ingest a variety of food items, from inorganic materials to a diversity of metazoans, including whole large prey. Total lipid content varied across species and was highest in Urticina sp. Phospholipids constituted the main lipid class in all three species, with consistently high levels of wax ester storage lipids. All sea anemones were also characterized by high proportions of mono and polyunsaturated fatty acids (MUFA and PUFA) as well as ω3 and ω9 FAs, and strikingly low proportions of 20:4ω6 (ARA). High values of 20:5ω3 (EPA), 20:1ω9 and 22:1ω11(13) evoke a diet centered on zooplankton, with notable particularities. For instance, Urticina sp. had the highest PUFA to saturated FAs ratio, indicative of carnivory. Overall, results suggest that the three sea anemone species occupy different niches in the spectrum of opportunistic polyphagous predation/feeding, with Urticina sp. relying chiefly on more energetic and larger prey and A. cristata targeting smaller zooplankton, foraminifera and particulate food, highlighting that large actinians play diverse roles in benthic food webs.