Abstract
Equilibrium binding affinity of yeast tRNA Phe for Escherichia coli poly(U)-programmed 70S ribosomal P-site was compared with corresponding affinities of several tRNA Phe 3′- and 5′-end-truncated derivatives, all containing the anticodon arm. Our findings strongly suggest that besides three 3′-terminal-CCA nucleotides (C74, C75 and A76), only the tRNA Phe anticodon arm (N28-N42) contains ribosomal P-site contact centers and that there are no such centers in the intermediate regions N1-N27 and N43-N73.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
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