Trk-like proteins during the post-hatching growth of the avian bursa of Fabricius.

Abstract

Neurotrophins are growth factors acting on responsive cells through membrane receptors identified as Trk tyrosine kinase proteins (A, B and C). Trks are present in the mammalian lymphoid organs, and indirect evidence suggests that they are also present in the avian bursa of Fabricius. This study was designed to analyze (a) the occurrence and localization of Trk proteins in the bursa of Fabricius; and (b) whether the post-hatching growth of the organ (from hatching to 75 days) involves cells expressing Trk proteins. We used pigeon bursae of Fabricius, and rabbit polyclonal antibodies against specific epitopes of TrkA, TrkB and TrkC. Cytokeratins and vimentin were studied in parallel with label non-lymphoid cells of the bursal follicles. Immunoreactivity (IR) for all assessed antigens was found in specific non-lymphoid cells. From hatching to 15 days, TrkB-like IR was found outside the follicles in cells localized beneath the follicle associated (FAE) and interfollicular (IFE) epithelium. Between 30-75 days TrkB-like IR labelled the medullary secretory dendritic cells (SDC). The density of SDC displaying IR increased up to 60 days. TrkA-like and TrkC-like IR was primarily observed in FAE and IFE, but also in the medullary reticular epithelial cells (REC) up to 15 days. The present results provide evidence of the occurrence, localization and post-hatching changes in Trk proteins in avian bursa of Fabricius. Trks were localized on non-lymphoid cells which participate in providing the adequate microenvironment for B lymphocyte maturation. Furthermore, the cell segregation in the expression of Trks suggests specific roles for their ligands in controlling the function of medullary SDC and REC, hence bursal lymphoid follicle physiology.