Abstract
Objective: Equine granulocytic anaplasmosis (EGA) caused by Anaplasma phagocytophilum is a disease that generally develop characteristic high fever and a number of changes in blood such as thrombocytopenia. The main transmission of A. phagocytophilum is hard ticks through intrastadially. The aim of this study was to determine the presence of A. phagocytophilum in horses in Muş province of Turkey by using molecular methods. Methods: In the present survey, blood samples were collected in Muş province from 93 horse. Commercial ELISA kit (IgG) and nested-PCR were performed. The DNA was extracted from blood and analysed by nested-PCR technique targeting 16S rRNA gene of A. phagocytophilum and then positive PCR products were sequenced. Results: The seroprevalence against A. phagocytophilum was 8.6% and 6 horses (6.4%) showed positive nested-PCR results. The sequences had 99.5 and 100% homology with the BLAST published A. phagocytophilum amplicons. Conclusion: To the best of our knowledge, this is the first molecular data of A. phagocytophilum in horses in Muş province of Turkey. This study also reveals that A. phagocytophilum isolates isolated from a small region are closely related to A. phagocytophilum isolated from a wide variety of hosts.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.