Abstract
Fifteen alfalfa varieties cultivated in Turkey were researched to compare the antioxidant potential of their seeds and find differences between the varieties. The total phenolic (TP) content, DPPH•, and ABTS•+ scavenging activities, and ferric-reducing antioxidant power (FRAP) of alfalfa seed methanol extracts were determined. TP contents of extracts were found between 37.03 and 54.04 mg GAE/g. ABTS assay results ranged between 0.100 and 0.158 mmol Trolox/g extract, and FRAP changed from 389.90 to 791.02 µmol Fe2+/g extract. The results demonstrated that the differences between the alfalfa varieties were significant (p < 0.01) in terms of analyzed characters.
Highlights
Alfalfa (Medicago sativa L.) is among the most cultivated forage legumes in Turkey and is generally recognized a suitable source of precious protein for animal feed (Gökkaya and Orak, 2021)
The present study revealed that the diversity of total phenolic content in the alfalfa seed extracts from fifteen varieties in comparison with their antioxidant activities
When the compared our results with recent research, the extraction yield with methanol from alfalfa seeds was found higher than the extraction yield from vetch seed which ranged from 6.15 to 9.70% (Orak, 2019) and mung bean seed which was determined as 10.7% (Orak et al, 2018)
Summary
Alfalfa (Medicago sativa L.) is among the most cultivated forage legumes in Turkey and is generally recognized a suitable source of precious protein for animal feed (Gökkaya and Orak, 2021). Due to the valuable chemical constituents of Medicago sativa L., which exhibit phytobiotic action, it could be used in folk medicine and phytotherapy treatments (Gawel et al, 2017). It has been reported that alfalfa is used as herbal medicine in traditional treatments in some countries such as Turkey, India, and America. To its high nutritional value, alfalfa has a history of medicinal uses among conventional medicinal plants in Middle Eastern countries, in China, in America, and in India (Krakowska et al, 2017). It has been reported that this plant has been used since antic time to treat fever, swelling, kidney stones, dysuria, and to relieve fluid retention by the Chinese people (Al-Dosari, 2012)
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