Tritium labelling of PACAP‐38 using a synthetic diiodinated precursor peptide

Abstract

In the interest of developing efficient methods for tritium labelling peptides, we here demonstrate the successful labelling of PACAP‐38 (pituitary adenylate cyclase‐activating polypeptide), a 38‐mer peptide, using a synthetic diiodinated PACAP‐38 precursor. In this example, we employ standard hydrogenation chemistry with the use of a heterogeneous palladium catalyst and carrier‐free tritium gas on a tritium manifold system. Copyright © 2011 John Wiley & Sons, Ltd.