Abstract
WRAB18, an ABA-inducible protein belongs to the third family of late embryogenesis abundant (LEA) proteins which can be induced by different biotic or abiotic stresses. In the present study, WRAB18 was cloned from the Zhengyin 1 cultivar of Triticum aestivum and overexpressed in Escherichia coli to explore its effects on the growth of E. coli under different abiotic stresses. Results suggested the enhanced exhibition of tolerance of E. coli to these stresses. Meanwhile, the WRAB18-transgenic tobacco plants were obtained to analyze the stress-related enzymatic activities of ascorbate peroxidase (APX), peroxidase (POD) and superoxide dismutase (SOD), and to quantify the content of malonaldehyde (MDA) under osmotic stress, high salinity, and low and high temperature stress. The activities of APX, POD and SOD in the transgenic tobacco lines were higher while the content of MDA was lower than those of WT lines. Moreover, plastid localization of WRAB18 in Nicotiana benthamiana plasma cells were found fusing with GFP. In addition, purified WRAB18 protein protected LDH (Lactate dehydrogenase) enzyme activity in vitro from various stress conditions. In brief, WRAB18 protein shows protective action behaving as a “molecular shield” in both prokaryotic and eukaryotic cells under various abiotic stresses, not only during ABA stress.
Highlights
Abiotic stresses were key restriction factors during the growth and production of crops
WRAB18 protein was expressed in the E.coli and examined by 12% SDS-PAGE stained with Coomassie Brilliant Blue R-250 (S2A Fig)
We performed in vivo and in vitro functional analysis to explore the protective effects of WRAB18 in different species cells under four kinds of stress
Summary
Abiotic stresses were key restriction factors during the growth and production of crops. Plants have developed numerous defense mechanisms to protect themselves under complex growth conditions through long-term evolutionary processes. These mechanisms are found at the cellular and molecular levels, such as changes in membrane lipid composition, production of new protein polymers, increased contents of sugars, organic acids, soluble proteins, proline, and ABA, and other corresponding changes necessary to resist or avoid adverse conditions [1]. LEA proteins represent one of these adaptions and are believed to play an important role in PLOS ONE | DOI:10.1371/journal.pone.0171340. LEA proteins represent one of these adaptions and are believed to play an important role in PLOS ONE | DOI:10.1371/journal.pone.0171340 February 16, 2017
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