Abstract
Five new oleanane-type triterpenoid saponins (1–5), together with 24 known saponins (6–29) were isolated from the fruit of Acanthopanax senticosus. Their structures were determined by extensive spectroscopic analysis, including 1D, 2D nuclear magnetic resonance (NMR), and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), in combination with chemical methods (acid hydrolysis). The neuroinflammation model was established by lipopolysaccharide (LPS)-induced BV2 microglia, and the neuroprotective effects of all compounds (1–29) were evaluated.
Highlights
Acanthopanax senticosus (Rupr. & Maxim.) Harms, commonly known as Ci Wu Jia or Siberian Ginseng, is a well-known traditional Chinese medicine widely distributed in the northeast of China
41 signals, of which 30 signals were assigned to a triterpene of oleanane skeleton, containing one carboxyl group, two olefinic carbons, two anomeric carbons, one downfield glycosylation-shifted oxygenated methine, a hydroxymethyl carbon, and six methyls. These observations implied that compound 1 might be an oleanane-type triterpenoid saponin
Data of 3 resembled those of 17, and the major difference was the substituent C-29 is changed from methyl to hydroxyl in compound 3, which was supported by the chemical shift δC 69.8 (C-20) and the heteronuclear multiple bond coherence (HMBC) correlation of the anomeric protons of H3-30 with C-19, C-20, and C-21 (Figure 2)
Summary
Acanthopanax senticosus (Rupr. & Maxim.) Harms, commonly known as Ci Wu Jia or Siberian Ginseng, is a well-known traditional Chinese medicine widely distributed in the northeast of China. Fr. VIII 6–(35) were purified by semi-preparative HPLC (MeOH/H2O 84%) to afford compounds 29 (2.8 mg), (7.7 mg), and (23.5 mg).
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