Triterpenes of Euonymus alatus and their cytotoxic activity

Abstract

observed in the mass spectra showed the molecular formula to be C 30 H 50 O 2 . The 1 H NMR spectrum revealed the presence of seven methyl groups: H 0.86 (3H, d, J = 6.7 Hz, H-23), 0.72 (3H, s, H-24), 0.87 (3H, s, H-25), 1.00 (3H, s, H-26), 1.07 (3H, s, H-27), 1.11 (3H, s, H-28); one of them was attached to a tertiary methyl, while the other six were attached to a quaternary carbon. The signal of the proton connected with the hydroxyl group carbon consisted of two parts: 3.34 (1H, d, J = 10.7 Hz) and 3.42 (1H, d, J = 10.7 Hz), while the signal of the secondary methyl carbon connected with the hydroxyl group appeared at C 71.9, showing that this hydroxyl group was attached to C-30 [11]. The 1 H and 13 C NMR (CDCl 3 ) data agreed with those of literature data [11, 12], so compound 1 was identified as 30-hydroxy-3-friedelanone. Compound 2 was obtained as a white amorphous powder (mp 272–273C), and the molecular ion peak ([M] + , m/z 442) observed in the mass spectra indicated that the molecular formula is C 30 H 50 O 2 . The 1 H and 13 C NMR (CDCl 3 ) data agreed with the literature data [11, 12], so compound 2 was identified as 29-hydroxy-3-friedelanone. Compound 3 was obtained as a white amorphous powder (mp 266–268C) and had the molecular C 30 H 50 O according to the mass spectra ([M + H] + , m/z 427). The 1 H and 13 C NMR (CDCl 3 ) data were in agreement with the literature [13, 14], so