TRITERPENE COMPOUNDS OF THE ABOVE GROUND ORGANS OF THE BUPLEURUM SCORZONERIFOLIUM WILLD. OF THE BAIKAL REGION FLORA

Abstract

Representatives of the genus Bupleurum L. in the composition of biologically active substances accumulate triterpene compounds (derivatives of α-amyrin and β-amyrin). Triterpene glycosides isolated from plants of the genus Bupleurum are called saikosaponinov, buplevrozidy, skorzonerozidy. Plants of the genus Bupleurum in the underground organs accumulate triterpene compounds in an amount of from 1 to 7%. Triterpene compounds of plants of the genus Bupleurum possess a hepatoprotective, antiviral, cytostatic effect. We have studied the triterpene compounds of the aerial organs of Bupleurum scorzonerifolium Willd. The material for the study was collected on the territory of the Irkutsk region (Russia). Isolation of triterpene compounds was performed with 70% ethyl alcohol. The compounds were identified by liquid chromatography with mass spectrometry and diode-matrix detection (UPLC-DAD-ESI-MS/MS). According to the results of the analysis, the content of 5 triterpene compounds was established: saikosaponinov A, B2, C, D, F. The isolated compounds are structurally derived from oleanan, in which glycosidation is observed at the C-3 position with α-L-rhinose, β-D-glucose and β-D-fucose. The spectrophotometric method determined the quantitative content of the amount of triterpene compounds in the above-ground organs of B. scorzonerifolium at different phases of the vegetation of the plant, by organs (in flowers, leaves, stalks), and also depending on the place of growth. It has been established that the maximum amount of the amount of triterpene compounds accumulates in the aboveground organs of B. scorzonerifolium during the flowering phase – 1.54±0.05%; the highest content of this group of compounds is noted in flowers – 1.70±0.06%; most of all, this group of compounds is collected by samples collected in the Ust-Horde Buryat District (1.96±0.06%). The aboveground organs of B. scorzonerifolium can be used as a source of triterpene compounds, the highest content of which is noted in the flowering phase. The study of triterpene compounds in the aboveground organs of B. scorzonerifolium was carried out for the first time.