Abstract
Separate (+) and (-) mating type cultures of Blakeslea trispora synthesized: (a) labeled trisporic acid B and trisporic acid C when incubated with labeled, partially purified extracts isolated from opposite mating type cultures; (b) unlabeled trisporic acids when incubated with labeled glucose and unlabeled extracts isolated from opposite mating type cultures; and (c) over 100-fold less trisporic acids when incubated with labeled extracts isolated from the same mating type cultures. Thus, separate (+) and (-) cultures synthesize mating type-specific precursors of trisporic acids.
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