Abstract
Heat shocks were applied to brook trout ova at different times after fertilization (range 5–25 min), with various temperatures (20–33°C) and durations (5–20 min). Incidence of triploid fry was determined from flow-cytometric analysis with blood samples. The best proportion of triploids and highest triploid yield were obtained with a shock treatment at 28°C for 10 min duration initiated 15 min after fertilization.
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