Abstract
The effectiveness of heat shocks in combination with electric shocks, derived from either alternating (AC) or direct sources (DC) (10 V), was compared with respect to their ability to induce triploidy in coho salmon. Electric shocks were applied to eggs for 2.5, 5.0 and 10 min duration, and for 10, 20 and 40 min post-fertilization, at 10 and 26°C. Control eggs received identical handling and heat treatments but without electric shocks. Treatment of developing embryos with AC currents was more effective in including triploidy than with DC current. AC shocks of 10 min duration, delivered to eggs held at 26°C, 40 min post-fertilization, produced 100% triploidization versus 70% and 15% observed in DC and control studies respectively. Ploidy states were verified by flow cytometric analyses.
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