Abstract
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
Highlights
Real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays
Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) typically relies on molecular testing of respiratory tract specimens, viral RNA can be detected in other specimens [1]
Real-time reverse transcription PCR protocols have been described for SARS-CoV-2, but most involve testing with multiple, singleplex reactions [2,3,4,5,6]
Summary
Author affiliations: Emory University, Atlanta, Georgia, USA Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. Real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
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