Abstract
p53, a tumor suppressor protein, has important roles in DNA repair, cell cycle and apoptosis, is a one of the key events in cancer development. Coix lacryma-jobi seed has been used as a food and traditional medicine plant with anti-oxidant, anti-cancer and anti-diabetic effects. In currently research, we identified the most potent p53-increasing compound among 4 compounds (1-4) found in Coix lacryma-jobi and demonstrated its molecular mechanism in MCF-7 cells. Among the four isolated compounds (1-4), triolein most increased p53. Triolein treatment induced p53, p21, p27 and Bax in MCF-7 cells. Moreover, triolein caused S phase arrest through suppression of CDK1, phopho-Rb and E2F1 in dose-dependent manner. We also observed the decreasing of DNA synthesis by triolein. These data suggest that triolein may induced cell cycle restart involve DNA synthesis and apoptosis pathway in MCF-7 cells. (Less)
Highlights
Cancer is a complex disease with multiple genetic and epigenetic changes
Chemotherapy is the most common types of cancer treatment. p53 plays an important role in cancer treatment mainly by increasing cell cycle arrest, senescence, DNA damage and apoptosis and interconnecting with many cellular functions[1,2]
We found that triolein from Coix seed extract induces p53 protein level in concentration-dependent manner in MCF-7 cells
Summary
Cancer is a complex disease with multiple genetic and epigenetic changes. Cancer cells proliferate without stopping and spread into surrounding tissues. P53 plays an important role in cancer treatment mainly by increasing cell cycle arrest, senescence, DNA damage and apoptosis and interconnecting with many cellular functions[1,2]. Previous studies demonstrated that Coix seed extract exhibits anti-inflammatory, protects against oxidative stress, and anti-cancer activities[5,6].
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