Abstract
Background Tripartite motif containing 58 (TRIM58), an E3 ubiquitin ligase, is reported as a suppressor gene in certain human tumors. However, the biological function of TRIM58 in osteosarcoma (OS) is still less identified. Methods In the present study, TRIM58 induced silencing and overexpression in OS cells using RNA interference (RNAi) and lentiviral-mediated vector, respectively. Cell proliferation profiles were analyzed using cell counting kit-8 (CCK-8) assay. Cell apoptosis profiles were determined using a flow cytometer. qRT-PCR and western blot were used to determine gene expression. Coimmunoprecipitation (Co-IP) assay was used to examine protein interaction. Results Our results demonstrated TRIM58 was downregulated in human OS tissues. Overexpression of TRIM58 remarkably suppressed the growth of OS cells and decreased glucose transportation and lactate secretion. These results indicated that TRIM58 involved in the regulation of energy metabolism in OS cells. Importantly, TRIM58 interacted with pyruvate kinase M2 (PKM2) in OS cells. Moreover, TRIM58 might inhibit the activity of PKM2 through enhancing its polyubiquitination in OS cells. Conclusions This analysis not only explored a deep understanding of the biological function of TRIM58 but also indicated its signaling pathway in OS cells.
Highlights
Osteosarcoma (OS) is one of the primary malignant tumors, which is caused by malignant osteoid production and osteoblastic differentiation [1]
All these results indicated that Tripartite motif containing 58 (TRIM58) was downregulated in OS tissues and cells
The polyubiquitination activity of pyruvate kinase M2 (PKM2) was much higher in oeTRIM58-transfected cells than that of oeNC-transfected cells. All these results demonstrated that TRIM58 interacted with PKM2 and enhanced its ubiquitination in OS cells
Summary
Osteosarcoma (OS) is one of the primary malignant tumors, which is caused by malignant osteoid production and osteoblastic differentiation [1]. TRIM58 is reported as a potential biomarker for colorectal cancer and identified as a tumor suppressor gene [5]. Tripartite motif containing 58 (TRIM58), an E3 ubiquitin ligase, is reported as a suppressor gene in certain human tumors. The biological function of TRIM58 in osteosarcoma (OS) is still less identified. Our results demonstrated TRIM58 was downregulated in human OS tissues. Ese results indicated that TRIM58 involved in the regulation of energy metabolism in OS cells. TRIM58 interacted with pyruvate kinase M2 (PKM2) in OS cells. TRIM58 might inhibit the activity of PKM2 through enhancing its polyubiquitination in OS cells. Is analysis explored a deep understanding of the biological function of TRIM58 and indicated its signaling pathway in OS cells Conclusions. is analysis explored a deep understanding of the biological function of TRIM58 and indicated its signaling pathway in OS cells
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