TRIM24 aggravates the progression of ovarian cancer through negatively regulating FOXM1 level.

Abstract

This study aims to uncover the biological functions of the feedback loop tripartite motif-containing 24 (TRIM24)/Forkhead Box M1 (FOXM1) in the pathological progression of ovarian cancer (OC) and the underlying mechanism. The expression levels of TRIM24 and FOXM1 in OC tissues and cells were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The potential correlation between TRIM24 level and clinical indexes of OC patients was analyzed. The Kaplan-Meier curves were depicted for evaluating the prognostic potentials of TRIM24 and FOXM1 in OC patients. The regulatory effects of TRIM24 and FOXM1 on proliferative, migratory, and invasive capacities of SKOV3 and OVCAR3 cells were assessed through functional experiments. The rescue experiments were performed to clarify the feedback loop TRIM24/FOXM1 in influencing the progression of OC. TRIM24 was upregulated in OC tissues and cells. The high level of TRIM24 was linked to higher rates of lymphatic and distant metastasis and worse survival in OC patients. The silence of TRIM24 attenuated proliferative, migratory, and invasive capacities of SKOV3 and OVCAR3 cells. FOXM1 level was negatively regulated by TRIM24, which was downregulated in OC. The low level of FOXM1 predicted worse survival in OC patients. Besides, the rescue experiments demonstrated that the feedback loop TRIM24/FOXM1 aggravated the malignant progression of OC. TRIM24 is upregulated in OC tissues, and closely linked to the occurrence of lymphatic and distant metastasis. Through negatively regulating FOXM1 level, TRIM24 aggravates the progression of OC.