Trigeminotectal and other trigeminofugal projections in neonatal kittens: an anatomical demonstration with horseradish peroxidase and tritiated leucine.

Abstract

The trigeminal projection to the superior colliculus in neonatal kittens was studied by using both anterograde and retrograde neuroanatomical tracing techniques. Trigeminothalamic observations also were made. In the first series of experiments, horseradish peroxidase was injected into the superior colliculus in kittens on the day of parturition and in adult cats. Retrogradely labeled cells were found throughout the contralateral sensory trigeminal complex: the greatest numbers of cells were concentrated in pars oralis, with fewer in the principal nucleus, and fewer still in pars interpolaris and pars caudalis. Thus, the distribution pattern of trigeminotectal cells in neonates is similar to that in adult animals. In the second series of experiments, we injected tritiated leucine into the rostral portion of the spinal trigeminal nucleus in neonatal kittens and adult cats and compared the laminar and spatial distribution of anterogradely transported label in the superior colliculus and thalamus. Terminal label was observed in both structures in animals as young as 1-2 days postpartum. The label in the superior colliculus was overwhelmingly contralateral and formed a tier of discontinuous patches in the stratum griseum intermediale and, in a more diffuse manner, in the stratum griseum profundum. Most of the patches were located in the rostral 80% of the superior colliculus and were 60-280 micron in width. Although the size of the patches was smaller in the neonates, their distribution was similar to that in adult cats. Thus, with the exception of the difference in patch size, the terminal pattern of trigeminotectal projections is essentially adultlike at birth. The dense pattern of contralateral terminal label in the arcuate division of the ventrobasal complex also was similar to that of the adult cat, as was the trigeminal projection to the supraoculomotor gray. These data indicate that the development of the spatial organization of a major ascending somatosensory pathway to the superior colliculus (and to the thalamus) is largely a prenatal event. It is likely that the further maturation of these systems during postnatal life is limited to fine changes in axonal terminals and synaptic formation within prenatally determined terminal territories. The in utero maturation of these trigeminofugal projections is necessary to enable the newborn kitten to utilize the perioral tactile cues necessary for early orientation and suckling behaviors.