Abstract
AbstractThe analysis of foods and feeds for the naturally occurringFusarium produced trichothecenes is reviewed. Each major step (extraction, purification and detection‐quantitation) is discussed. Although none of the extraction solvents has been thoroughly evaluated, aqueous metanol is the preferred system in most of the published procedures. The sample extracts are generally purified by liquid‐liquid partitions followed by silica gel column and/or preparative thin layer chromatography (TLC). Both thin layer and gas liquid chromatography (GLC) are used for the detection and quantitation of the trichothecenes. The detection limit for the GLC procedures has not been determined in most of the reported methods; however, T‐2 toxin, diacetoxyscirpenol, deoxynivalenol, and neosolaniol have been detected in the 25–100 ng/g range.
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