Trichogenic Silver-Based Nanoparticles for Suppression of Fungi Involved in Damping-Off of Cotton Seedlings.

Shimaa A Zaki,Anu Kalia,Amal A Asran,Mohamed M Hassan,Fawziah M Albarakaty,Kamel A Abd-Elsalam,Salama A Ouf

doi:10.3390/microorganisms10020344

Abstract

Mycogenic silver nanoparticles (AgNPs) produced by some biocontrol agents have shown the ability to inhibit the growth of numerous plant pathogenic fungi, which may be a unique method of disease management. This study describes the extracellular production of AgNPs by Trichoderma harzianum. The size, shape, charge, and composition of the AgNPs were subsequently studied by UV-visible spectroscopy, DLS, zeta potential, TEM, SEM, and EDX, among other methods. The AgNPs had sizes ranging from 6 to 15 nm. The antifungal activities of bio-synthesized AgNPs and two commercial fungicides (Moncut and Maxim XL) were tested against three soil-borne diseases (Fusarium fujikuroi, Rhizoctonia solani, and Macrophomina phaseolina). Cotton seedling illnesses were significantly reduced under greenhouse settings after significant in vitro antifungal activity was documented for the control of plant pathogenic fungi. The use of biocontrol agents such as T. harzianum, for example, may be a safe strategy for synthesizing AgNPs and using them to combat fungus in Egyptian cotton.

Highlights

Accepted: 26 January 2022Cotton (Gossypium barbadense L.), which belongs to the family Malvaceae, is considered one of Egypt’s main cash crops
Soil samples were taken from healthy cotton root rhizosphere soils and stored at 4 ◦ C
This study revealed a high concentration of AgNPs (68.60 percent), followed by oxygen, carbon, and nitrogen, indicating that extracellular organic moieties from mycelial free cell filtrate were adsorbed on the nanoparticles’ surface [21]

Summary

Introduction

Accepted: 26 January 2022Cotton (Gossypium barbadense L.), which belongs to the family Malvaceae, is considered one of Egypt’s main cash crops. In the preponderance of Egypt’s cotton-growing zones, R. solani was discovered to be the most important cause of cotton damping-off. A pathogenicity assay of R. solani belonging to different Anastomosis groups (AGs), i.e., Ag2-2, AG-4, AG-5, and AG-7, under greenhouse conditions revealed that most of the virulent isolates caused pre-emergence damping-off [2,3]. All of these isolates were extremely pathogenic, causing 100 % death in the pre-emergence stage [2,3,4,5]

Methods

Discussion

Conclusion