Abstract
AbstractThe activity and denaturation extent of cellulase from Trichoderma reesei (E.C. 3.2.1.4) was investigated in three representative N,N-dimethylethanolammonium akylcarboxylate ionic liquids. Significant cellulase activity and absence of enzyme unfolding was found in all concentrations of N,N-dimethylethanolammonium acetate (DMEAA), including the pure liquid. Activities in 20% and 40% (v/v) solutions of DMEAA were equal to citrate buffer controls. Lower enzymatic activities and denaturation were observed in solutions of the corresponding formate and octanoate ionic liquids, although cellulose hydrolysis still proceeded at a substantial rate. The results provide the first proof-of-principle that cellulose can be enzymatically hydrolyzed in the presence of high ionic liquid concentrations.
Highlights
Among the various raw biomaterials that will form part of the emerging biomass-based economy, cellulose will play a central role due to its large annual production via photosynthesis,[1] and its potential to be used for applications in the biofuels, fine chemicals, fibre, and polymer industries.[2]
Lower enzymatic activities and denaturation were observed in solutions of the corresponding formate and octanoate ionic liquids, cellulose hydrolysis still proceeded at a substantial rate
While the hydrophobic effect that increases protein stabilization is absent in organic ionic liquids (ILs), one advantage to biocatalysis in ILs versus aqueous buffers is the longer activity of enzymes in ILs, thought to be from the slow breaking and remaking of hydrogen bonds in the non-aqueous media
Summary
Among the various raw biomaterials that will form part of the emerging biomass-based economy, cellulose will play a central role due to its large annual production via photosynthesis,[1] and its potential to be used for applications in the biofuels, fine chemicals, fibre, and polymer industries.[2]. Significant cellulase activity and absence of enzyme unfolding was found in all concentrations of N,N-dimethylethanolammonium acetate (DMEAA), including the pure liquid.
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