Abstract
Trichloroethylene (TCE) is a volatile organic compound that is widely used in industries and potential source of environmental contamination. The aims of this study were focused on the effects of TCE on cellular viability and evaluation of critical markers like caspase-3 and glutathione (GSH) in human lymphocytes. The experiments were studied in thirty volunteers. The TCE concentrations of 0.002, 0.004, 0.008, 0.016 and 0.032 mM/L were treated in human lymphocytes for 48 h. Lymphocytes were cultured and effect of TCE on viability of lymphocytes was studied by MTT assay. The expression of caspase-3 in TCE treated lymphocytes was studied by ELISA and the concentration of glutathione in TCE treated lymphocytes was studied by colorimetric technique. The results showed that, in the experimental groups at various TCE concentrations had lower cellular viability than the control group (p<0.05). The caspase-3 concentration was increased when TCE concentration increased (p<0.05). The glutathione concentration was decreased when TCE concentration increased (p<0.05). It is indicated that TCE effected on cellular viability. The expression of caspase-3 enzyme and glutathione concentrations were changed by TCE toxicity in lymphocytes.
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