Trichinella spiralis: X-ray analysis of the nurse cell-muscle larva complex after exponential growth of the larva.

Abstract

The energy-dispersive X-ray analysis (EDXA) method was employed to determine how the exponential growth of the Trichinella spiralis larva affect the concentration of some metabolically active elements such as phosphorus, sulfur, sodium, chlorine, potassium, and calcium in host myocytes as well as inside the nurse cell-muscle larva complex. Two observations are noteworthy. First, the capsule of the complex reveals the highest Na+ and Cl-concentrations. We would suggest that these high Na+ and Cl-concentrations could be a part of the capsule-localized transport mechanisms covering the metabolic demands of the larva. Second, a substantial increase in the P concentration in the cells inside the complex, i.e., the nurse cell, somatic muscle of the larva, and larval stichosome was found. We postulate that energy metabolism in the nurse cell is entirely dependent on the metabolic requirements of the larva and that they remain together as one functional unit.