TRIB1 and TRPS1 Variants, GxG and GxE Interactions and Serum Lipid Levels, the Risk of Coronary Heart Disease and Ischemic Stroke

Abstract

Background: Little is known about the association of the tribbles pseudokinase 1 (TRIB1) and transcriptional repressor GATA binding 1 (TRPS1) variants and the gene-gene (GxG) and gene-environment (GxE) interactions with serum lipid levels, the risk of coronary heart disease (CHD) and ischemic stroke (IS) in the Guangxi Han population. The present study was performed to examine these association. Methods: Genotyping of the rs2954029, rs2980880, rs10808546, rs231150, rs2737229 and rs10505248 SNPs was performed in 625 controls and 1146 unrelated patients (CHD, 593 and IS, 553) by polymerase chain reaction and restriction fragment length polymorphism, and then confirmed by direct sequencing. Findings: The SNPs were associated with increased risk of CHD (rs2954029 and rs231150) and IS (rs2954029). Several SNPs in controls were associated with different lipid parameters. The rs2954029TA/AA-age (> 60 year) interaction increased the risk of CHD, whereas the rs10808546CT/TT-drinking interaction decreased the risk of IS. The haplotype of A-C-C was associated with an increased risk of CHD and IS. The haplotype of A-T-C was associated with an increased risk of CHD. The interactions of rs2954029-rs231150 had an increased risk of both CHD and IS. Interpretations: Several SNPs in the TRIB1 and TRPS1 were associated with increased risk of CHD and IS, and associated with serum lipid parameters in controls. The GxG and GxE interactions on serum lipid levels, the risk of CHD and IS were also observed. Funding: The work was supported by the Science Foundation of Guangxi Returned Oversea Scholars (No. 0991004) and the National Natural Science Foundation of China (No. 81460169). Declaration of Interest: The authors declare no competing financial interests. Ethical Approval: This study was approved by the Ethics Committee of, the First Affiliated Hospital, Guangxi Medical University, and written informed consent was obtained from each participant before data collection. The reported investigations were in accordance with the principles of the Declaration of Helsinki.