Abstract
Triazine-resistant mutants have been isolated in photomixotrophic cell cultures of Nicotiana plumbaginifolia. Triazine herbicides inhibit photosynthesis and cause extensive photodestruction of chloroplasts (bleaching) in sensitive plants. Selection was based on the greening ability of the resistant cells in the presence of 10-4 M terbutryn, under normal culture conditions, but in a medium containing a low sugar concentration. In the mutant plants, as compared to wild type, two to three orders of magnitude higher concentrations of triazines resulted in inhibition of photosynthetic electron transport and greening. The resistance was inherited maternally.
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