Abstract
Ten sexually mature rabbit bucks of (NZW) were used in this study. The present study aimed to investigate the effect of some diluents and differents regimes of dilution and preservation on the viability of rabbit semen . Semen was collected by an artificial vagina and evaluated for volume (ml), individual motility (%); concentration of spermatozoa (ml × 106); percentage of alive spermatozoa and total abnormalities by conventional technique. Pooled semen sample was divided into three parts, the first part was extended in Tris – egg yolk (pH 6.9), the second part was extended in Tris-egg yolk (pH 7.5) and the third part was extended in sodium chloride (pH 7.0). Each part again was subdivided into three portions; the first portion was incubated at 37˚C. for 3 hr.; The second portion was kept at 5 oC. for 48 hr. and the third portion was frozen in French straws (0.25 ml.) and stored in liquid nitrogen ( -196˚C). Our results revealed that the percentage of alive spermatozoa displayed a significant increase (P<0.05) at 37 oC in sodium chloride extender than the other extenders, while there were no significant differences in individual sperm motility in the three extenders at 37 oC. .On the other hand the individual sperm motility signed a significant increase (p < 0.05) in Tris-egg yolk extender with pH 6.9 than the other extenders at 5˚C. The post thawing motility was significantly higher (P<0.05) in Tris-egg yolk extended semen with pH 6.9 than the other extended semen. The extended semen with sodium chloride reported a highly significant percentage in acrosomal defects than the other extended semen with different pH.
Highlights
Artificial insemination is a biotechnological tool used for genetic improvement
Our results revealed that the percentage of alive spermatozoa displayed a significant increase (P
Semen was collected twice weekly for Six weeks using an artificial vagina according to the method described by Evans and Maxwell, (1987).Semen ejaculates were individually evaluated microscopically and only ejaculates exhibiting active sperm motility were pooled and extended with different extenders having different pH (Tris-egg yolk pH 7.5, Tris-egg yolk pH 6.9 and Sodium chloride pH 7)
Summary
Artificial insemination is a biotechnological tool used for genetic improvement. It is used in all animal species with many purpose including production, planning and control breeding which is associated to maximize enterprise profitability. Rabbits are not the exception where the artificial insemination is applied with the same objectives. A limitation factor for rabbit artificial insemination spread in commercial level is related to semen preservation. Fresh diluted semen has been used but its quality can be maintained only for a short period of time. Alvarino et al (1996) proved that semen storage for short time less than 48 hours at temperature between 5 ̊C25 ̊C was commonly adopted in practice Fresh diluted semen has been used but its quality can be maintained only for a short period of time. Alvarino et al (1996) proved that semen storage for short time less than 48 hours at temperature between 5 ̊C25 ̊C was commonly adopted in practice
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